In a paper by J. Hall, U. Bexell, J. S. Fletcher, S. Canovic and P. Malmberg, Oxide scale cross-sections of CeO2 coated FeCr based solid oxide fuel cell interconnect materials were examined using secondary ion mass spectrometry (SIMS) depth profiling. Secondary ion mass spectrometry sputter depth profiles using different ion sources; 69Ga+, Bi3+/Cs+ and C60+/C602+ were compared with TEM oxide scale cross-section and field emission gun–Auger electron spectroscopy depth profiling. The C60cluster ion depth profiles were less sensitive to type of matrix and gave the best correspondence to the TEM cross-section.
Read more in Materials at high temperatures
Check out this review by Jörg Hanrieder, Per Malmberg and Andrew G. Ewing on neuroproteomics in BBA Proteins and Proteomics .
- •Imaging MS allows for spatial protein and peptide profiling at high molecular specificity.
- •Recent developments push the limits in terms of spatial resolution, sensitivity and specificity.
- •A concise overview of IMS based approaches for protein and peptide imaging is presented.
- •Technical concepts and developments of IMS are discussed.
- •Implementations of IMS for biomedical applications in neuroscience are presented.
An exiting new paper on demonstrating the advantages of using gas cluster ion beams (GCIBs) in combination with trifluoracetic acid (TFA) vapor exposure for the imaging of lipids in mouse brain sections. There is an optimum exposure to TFA that is beneficial for increasing high mass signal as well as producing signal from previously unobserved species in the mass spectrum. Cholesterol enrichment and crystallization on the sample surface is removed by TFA exposure uncovering a wider range of lipid species in the white matter regions of the tissue, greatly expanding the chemical coverage and the potential application of TOF-SIMS imaging in neurological studies. Ar4000+ (40 keV) in combination with TFA treatment facilitates high resolution, high mass imaging closing the gap between TOF-SIMS and matrix-assisted laser desorption ionization (MALDI).
Read the paper by Tina B. Angerer, Masoumeh Dowlatshahi Pour, Per Malmberg, and John S. Fletcher in Analytical Chemistry.
Hanrieder et al. published their paper on elucidation of spatial lipid dynamics in post mortem spinal cord of patients suffering from Amyotrophic Lateral Sclerosis. The data reveal an ALS associated increase of proinflammatory lipid mediators and metabolites in confined regions of the ventral spinal cord. The data give insight in potential mechanisms underlying motor neuron degeneration in ALS. The paper is available online at Nature’s Scientific Reports: www.nature.com/srep/2014/140612/srep05266/full/srep05266.html
Next week the NCIMS will present several posters at ASMS2014 (62nd ASMS Conference on Mass Spectrometry and Allied Topics) in Baltimore. Come meet us there for new insights into imaging mass spectrometry! Check out our posters in the “Imaging MS: Small Molecules and Drugs”, “Imaging MS: Method Development II” and “Imaging MS: Instrumentation”-sessions.
Hanrieder et al. at the NCIMS just published a study on ToF SIMS based molecular imaging for studying effects of neonatal exposure to the environmental toxin BMAA. In an animal model, neurochemical long term changes in the hippocampus upon neurotoxin exposure were studied. The results show BMAA induced metabolite regulations in distinct hippocampal regions that are mainly associated with protein aggregation , neuronal degeneration and astrogliosis. The Paper is now online at ACS Chemical Neuroscience http://dx.doi.org/10.1021/cn500039b
Tina Angerer’s new paper “High energy gas cluster ions for organic and biological analysis by time-of-flight secondary ion mass spectrometry” is now online at IJMS.
Professor Andrew Ewing and the NCIMS has been awarded a grant of 37 million from the Knut and Alice Wallenberg Foundation for the opportunity to complement our techniques with a nanoSIMS. The grant is being awarded to the project “The Imaging metabolomics and lipidomics infrastructure”, a project with a focus on imaging life’s smallest constituents and to understand and interpret these elements in the unique environment of cells and tissues.
Now, our capabilities will be complemented by adding a high resolution imaging technique – a Cameca NanoSIMS. The NanoSIMS is a key component here and will fill research needs in many scientific fields such as medicine, environmental, and material science in terms of access to this form of high resolution molecular imaging”, says Professor Andrew Ewing of the Department of Biochemistry and Molecular Biology, University of Gothenburg and Chalmers University of Technology.
Read more at the University webpage .
Maximising the potential for bacterial phenotyping using time-of-flight secondary ion mass spectrometry with multivariate analysis and tandem mass spectrometry by Wehrli et al. is now available online!